Aminated poly(L-histidine) as new pH-sensitive DNA carrier.

Abstract

The endosomal escape of the polyion complex between DNA and polycation is one of important factors for efficient gene expression. Here we have examined whether the poly(L-histidine) with several aminoethyl groups, i.e., aminated poly(L-histidine), possessed the pH-sensitive properties as DNA carrier. The polyion complex formation of the aminated poly(L-histidine) with DNA was affected by the endosomal pH of the medium, leading to significant transfection activity.