Fluorescent mRNA molecules offer a wide range of applications for studying capping/decapping reactions, translation, and other biophysical studies. Furthermore, fluorescent tags prove invaluable for tracking RNA molecules in cells. Here, we describe an efficient synthesis of a fluorescent cap analog, anthranioyl-GTP, its purification, and in vitro cap labeling of transcribed mRNA catalyzed by the recombinant vaccinia capping enzyme to produce anthranioyl-m7GpppG-capped RNA.
CITATION STYLE
Domashevskiy, A. V., Rodriguez, D. J., Gunawardana, D., & Goss, D. J. (2016). Preparation of functional, fluorescently labeled mRNA capped with anthraniloyl-m7GpppG. In Methods in Molecular Biology (Vol. 1428, pp. 61–75). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3625-0_4
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