LC/MS n for glycoprotein analysis: N-linked glycosylation analysis and peptide sequencing of glycopeptides

Abstract

Liquid chromatography/multiple-stage mass spectrometry (LC/MS n) is an effective means for the site-specific glycosylation analysis of a limited quantity of glycoproteins, such as gel-separated proteins. Generally, a tryptic digest of the glycoprotein is separated by reversed-phase LC, and peptide sequencing and glycosylation analysis are achieved with on-line MS n. In this chapter, a protocol for the LC/MS/MS/MS of a proteolytic digest of a gel-separated glycoprotein is described. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.