Regulation of luteinizing hormone secretion in prepubertal heifers fed an energy-deficient diet

Abstract

The effects of feeding a diet restricted in energy on the endocrine mechanisms regulation LH secretion in prepubertal heifers were examined. On Day 0, thirty heifers were assigned to be either ovariectomized (OVX; n = 10), ovariectomized and administered an estradiol implant (OVXE; n = 10), or to remain ovary-intact (INT, n = 10). Five heifers each were then assigned to be fed either a control (C) or a two-phase, low-to-high energy (L), dietary treatment. The C diet was formulated to support weight gains of 1.1 kg/day throughout the experiment (Day 0 - 197). Heifers receiving the L treatment were provided a diet restricted in energy (33% of C diet) from Day 0 - 127 followed by a diet similar to that fed to heifers receiving the C treatment from Day 128 - 197. Secretion of LH increased rapidly following ovariectomy in C-OVX and L-OVX heifers. During the period of energy restriction, LH pulse frequency was reduced, and mean LH concentration, pulse amplitude, and pituitary response to LHRH were greater in L-OVX than in C-OVX heifers. In L-OVXE and L-INT heifers, secretion of LH was low and unchanged during the period of dietary energy restriction. In contemporaneous treatment groups fed the C diet (C-OVXE and C-INT), frequency of LH pulses increased gradually during this period and C-INT heifers attained puberty on Day 121 ± 18. Upon switching heifers in the L-OVX, L-OVXE, and L-INT treatments to the C diet, all characteristics of LH secretion changed markedly within 14 days to levels similar to those detected in the C treatments. Day of puberty for heifers in the L-INT treatment (141 ± 6) was not different from Day of puberty for heifers in the C-INT treatment. It is concluded that dietary energy restriction sustained inhibition of LH secretion in OVXE heifers and in INT heifers. Additionally, dietary energy restriction inhibited LH secretion independent of ovarian factors in prepubertal heifers.