Enzymatic Dehydrogenation of Testosterone Coupled to Pyruvate Reduction in a Two‐Phase System

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Abstract

Following previous work, the enzymatic dehydrogenation of testosterone into 4‐androstene‐3,17‐dione is carried out in water and organic solvent system. The organic solvent contains the steroid and the water phase contains the β‐hydroxysteroid dehydrogenase together with pyruvate and lactate dehydrogenase. In such a system, the reduced coenzyme formed by dehydrogenation of the steroid is oxidized again by pyruvate and the conversion of testosterone is essentially stoichiometric with the pyruvate added. The effect of several variables on the equilibrium and kinetic behaviour of the system has been analyzed. The results obtained provide another good example of the advantages which may be obtained in carrying out enzyme reactions with poorly water‐soluble substrates in emulsions of water and organic solvents. Copyright © 1974, Wiley Blackwell. All rights reserved

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Cremonesi, P., Carrea, G., Ferrara, L., & Antonini, E. (1974). Enzymatic Dehydrogenation of Testosterone Coupled to Pyruvate Reduction in a Two‐Phase System. European Journal of Biochemistry, 44(2), 401–405. https://doi.org/10.1111/j.1432-1033.1974.tb03497.x

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