Biochemical analyses of lipids deposited on silicone hydrogel lenses

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Abstract

Purpose: This study was performed to determine the levels of lipids deposited on in vivo worn silicone hydrogel lenses. Methods: Three silicone hydrogel materials, galyfilcon A, senofilcon A, and asmofilcon A, were worn for 2 weeks by 35 normal subjects. Total lipid deposition was determined by the sulfo-phospho-vanillin reaction. Cholesterol was estimated by a colorimetric probe through enzymatic oxidation. Phospholipid level was estimated by determining phosphorus with ammonium molybdate through enzymatic digestion. Results: The total lipid content recovered from galyfilcon A, senofilcon A, and asmofilcon A was 32.9 ± 33.8, 42.1 ± 14.0, and 36.6 ± 31.9 μg/lens, respectively. The cholesterol content recovered from galyfilcon A, senofilcon A, and asmofilcon A was 26.2 ± 26.9, 28.6 ± 19.4, and 31.1 ± 21.1 μg/lens, respectively. There were no statistically significant differences in total lipids and cholesterol among the contact lens types. However, the quantity of phospholipid recovered from the asmofilcon A (7.0 ± 5.5 μg/lens) lenses was significantly higher than from galyfilconfilcon A (2.4 ± 0.8 μg/lens) lenses (p < 0.05, Mann-Whitney test). Conclusions: The quantity of total lipid and cholesterol deposited on the 3 silicone hydrogel lenses tested did not differ. However, there were significant differences in the amounts of phospholipidd eposited among the 3 silicone hydrogel lenses, of which clinical significance should be explored in the future study. © 2010 Spanish General Council of Optometry. Published by Elsevier España, S.L. All rights reserved.

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APA

Hatou, S., Fukui, M., Yatsui, K., Mochizuki, H., Akune, Y., & Yamada, M. (2010). Biochemical analyses of lipids deposited on silicone hydrogel lenses. Journal of Optometry, 3(3), 164–168. https://doi.org/10.1016/S1888-4296(10)70023-6

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