Extracellular laccases from submerged cultures of Coriolus versicolor BKM F-116, Panus tigrinus 8/18, Phlebia radiata 79 (ATCC 64658), Plebia tremellosa 77-51 and from cultures of Pa. tigrinus 8/18, Ph. radiata 79 and Agaricus bisporus D-649 grown on wheat straw (solid-state fermentation) were purified. All enzymes from submerged cultures had a blue colour and characteristic absorption and EPR spectra. Laccases from the solid-state cultures were yellow-brown and had no typical blue oxidase spectra and also showed atypical EPR spectra. Comparison of N-terminal amino acid sequences of purified laccases showed high homology between blue and yellow-brown laccase forms. Formation of yellow laccases as a result of binding of lignin-derived molecules by enzyme protein is proposed.
Leontievsky, A. A., Vares, T., Lankinen, P., Shergill, J. K., Pozdnyakova, N. N., Myasoedova, N. M., … Hatakka, A. (1997). Blue and yellow laccases of ligninolytic fungi. FEMS Microbiology Letters, 156(1), 9–14. https://doi.org/10.1016/S0378-1097(97)00393-5