A 'branched' mechanism of the reverse reaction of yeast glutathione reductase An estimation of the enzyme standard potential values from the steady-state kinetics data

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Abstract

The reduced glutathione-linked NADP+ reduction, catalyzed by yeast glutathione reductase, follows a 'sequential' or 'ping-pong' mechanism at high or low NADP+ concentrations, respectively. The pattern of the NADPH and NADP+ cross-inhibition reflects not only the competition for the binding site, but the shift of the reaction equilibrium as well. A 'branched' scheme of the glutathione reductase reaction is presented. The enzyme standard potential (-255 mV, pH 7.0) was estimated from the ratio of the NADPH and NADP+ rate constants corresponding to the ping-pong mechanism. © 1989.

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Rakauskien, G. A., Čnas, N. K., & Kulys, J. J. (1989). A “branched” mechanism of the reverse reaction of yeast glutathione reductase An estimation of the enzyme standard potential values from the steady-state kinetics data. FEBS Letters, 243(1), 33–36. https://doi.org/10.1016/0014-5793(89)81212-8

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