Patient tumor tissue processing is an important step in the generation of clinically relevant specimens for in vitro and in vivo studies. Proper disassociation and tissue sample cleanup is a multistep, time-consuming process that ultimately effects the generation of patient derived xenografts and neurosphere cultures. Here we describe a detailed protocol on how to process and disassociate patient glioma tissue and subsequent steps on orthotopic implantation and in vitro generation of neurospheres.
CITATION STYLE
Gosa, L., Ta, L., & Nathanson, D. A. (2019). Processing of primary patient tumors and subsequent generation of primary cell lines. In Methods in Molecular Biology (Vol. 1897, pp. 425–431). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8935-5_34
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