Small RNAs, defined as noncoding 20-30-nt-long RNAs, are instrumental regulators of cellular processes in most eukaryotes. In this chapter we describe three methods for extracting small RNA from cells: a general method, one plant specific and a third particular to conifers. Further, protocols are given for the analysis and quantification of small RNAs using polyacrylamide gel-based approaches. A native streptavidin gel-shift assay, useful for measuring the relative amounts of multiple small RNAs simultaneously, is presented. To further characterize small RNAs biochemically, a sodium periodate assay probing for 2′, 3′ hydroxyl groups on the 3′ terminus of small RNAs is outlined. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Ebhardt, H. A., Ovando, M. O., & Unrau, P. J. (2012). Isolation and biochemical analysis of plant small RNAs. Methods in Molecular Biology, 894, 223–239. https://doi.org/10.1007/978-1-61779-882-5_15
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