Isolation of mouse hepatocytes

Abstract

Primary hepatocyte cultures better re flect the properties of the liver in vivo than do cell lines derived from the liver. Here we describe a method for the isolation and culture of mouse primary hepatocytes. The cells are viable, can be transfected by DNA, and retain key properties of liver cells such as the induction of cytochrome P450 gene expression by drugs such as phenobarbital. © Springer Science+Business Media New York 2013.