ImmunoRNases represent a highly attractive alternative to conventional immunotoxins for cancer therapy. Quantitative production of immunoRNases in appropriate expression systems, however, remains a major challenge for further clinical development of these novel compounds. Here we describe a method for high-level production and purification of a fully functional immunoRNase fusion protein from supernatants of stably transfected mammalian cells.
CITATION STYLE
Krauss, J., Exner, E., Mavratzas, A., Seeber, S., & Arndt, M. A. E. (2009). High-level production of a humanized immunoRNase fusion protein from stably transfected myeloma cells. Methods in Molecular Biology (Clifton, N.J.), 525. https://doi.org/10.1007/978-1-59745-554-1_24
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