Resection of a DNA Double-Strand Break by Alkaline Gel Electrophoresis and Southern Blotting

Abstract

Generation of 3′ single-stranded DNA (ssDNA) at the ends of a double-strand break (DSB) is essential to initiate repair by homology-directed mechanisms. Here we describe a Southern blot-based method to visualize the generation of ssDNA at the ends of site-specific DSBs generated in the Saccharomyces cerevisiae genome.