HCV infection provides a classic example of the phenomenon of quasispecies. Because several lines of investigation support the contribution of quasispecies to HCV's capacity to maintain a persistent infection, adequate characterization of the quasispecies is important. The hypervariable region 1 (HVR1) of the E2 glycoprotein has been particularly well studied in this regard. We present here a rapid method for characterizing the HVR1 quasispecies, based on in vitro coupled transcription/translation of the amplicons, followed by mass spectrometry of the resulting peptide mix.
CITATION STYLE
Yea, C., Ayers, M., & Tellier, R. (2009). Characterization of HCV quasispecies in the hypervariable region 1 (HVR1) by in vitro translation and mass spectrometry. Methods in Molecular Biology (Clifton, N.J.), 510, 73–81. https://doi.org/10.1007/978-1-59745-394-3_6
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