Objective: To investigate the roles of miR-34a in progression and chemoresistance of glioma cells. Results: Quantitative real-time PCR analysis showed that miR-34a level was lower, but PD-L1 expression level was higher in glioma tissue specimens compared with normal brain tissues and their expression levels were negatively correlated. Ectopic expression of miR-34a inhibited glioma cell proliferation, promoted cell cycle arrest in G1/S phase and cell apoptosis. Additionally, miR-34a/PD-L1 axis was again confirmed and co-expression of PD-L1 with miR-34a mimics attenuated the effects of miR-34a on cell proliferation and apoptosis in glioma cells. Importantly, PD-L1 overexpression resulted in chemoresistance in glioma cells, this effect was attenuated by miR-34a overexpression. Conclusions: miR-34a inhibits glioma cells progression and chemoresistance via targeting PD-L1.
CITATION STYLE
Wang, Y., & Wang, L. (2017). miR-34a attenuates glioma cells progression and chemoresistance via targeting PD-L1. Biotechnology Letters, 39(10), 1485–1492. https://doi.org/10.1007/s10529-017-2397-z
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