Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5′-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells. © 1984 Springer-Verlag.
CITATION STYLE
Boeke, J. D., La Croute, F., & Fink, G. R. (1984). A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. MGG Molecular & General Genetics, 197(2), 345–346. https://doi.org/10.1007/BF00330984
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