Variation Of Andrographolite Conten From Sambiloto (Andrographis paniculata Ness) Which Grow In Various Regions

Abstract

Sambiloto is used as a hepatoprotector, enhancing the immune system, antidiabetic, anti-inflammatory, antiomalaria, antidiarrheal and many more. One of the main ingredients of the bitter plant is andrographolite. This plant is widely spread throughout Indonesia ranging from lowlands to high plains with various types of soil and altitude. The existence of this fairly wide distribution can give effect to the content of andrographolite, so that in this study will be studied about variations in andrographolite levels source  from various regions. The results of this study will be used as a quality reference for treatment based on andrographolite. Samples of bitter plants are taken from various regions. Furthermore, parts of plants that are above the ground are separated between leaves, twigs and stems. All samples were dried in an oven at 60 degrees Celsius. Pollinated samples were then sieved. Samples were extracted with ethanol overnight and then analyzed their andrographolite levels with TLC-densitometry.The results of the analysis of andrographolite levels showed that bitter from various regions have different levels. Tthe organs of plants also show different levels of andrographolite as well. Part of the leaf organ is the part that contains the most andrographolite.