Immunofluorescence of histone proteins

Abstract

The identification of a vast array of posttranslational modifications of histone proteins during cell cycle, repair, replication, and transcription has created the challenge of determining structure-function relationships for individual modifications and combinations of modifications. Some of this information can be gathered from indirect immunofluorescence, where the location and cell cycle relationships can be readily identified. Here we present an immunofluorescence protocol that is adapted for the use in histone modifications.