Structural and redox behavior of oxyvita™, a zero-linked polymeric hemoglobin: Comparison with natural acellular polymeric hemoglobins

13Citations
Citations of this article
6Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

A zero-linked polymeric hemoglobin (OxyVita Hb) has been developed for application as an acellular therapeutic hemoglobin-based-oxygen-carrier (HBOC). For effective and safe oxygen binding, transport and delivery, an HBOC must meet essential molecular requirements related to its structural integrity and redox stability. OxyVita™ is a super polymer possessing an average M.wt. of 17 × 106 Da. Structural integrity was determined by unfolding studies of OxyVita™ in the presence of increasing concentrations of urea. The unfolding midpoints (D1/2) of different preparations of OxyVita™ (solution and powder forms) were compared to Lumbricus Hb (LtHb) and Arenicola Hb (ArHb), natural acellular polymeric hemoglobins, which are serving as models for an effective and safe acellular HBOC. Reduction studies of OxyVita Hb using endogenous reducing agents were also investigated. Results from these studies indicate that: 1) OxyVita Hb exhibits greater resistance to conformational change than either LtHb or ArHb in the reduced (oxyHb) state; and 2) the reduction of met OxyVita Hb to oxyHb occurs slowly in the presence of either ascorbic acid (70% reduction in 560 min.) or β-NADH (40% reduction in 90 min.). These studies provide consistent evidence that OxyVita Hb possesses physiochemical properties that exhibit structural integrity and redox behavior necessary for functioning as an effective and safe HBOC within clinical applications. These results are in agreement with observations made by other investigators as to the reduction in heme-loss of OxyVita Hb, essential for the reversible binding/release of molecular oxygen within the circulatory system. © Informa UK Ltd.

Cite

CITATION STYLE

APA

Harrington, J. P., Orlig, K., Zito, S. L., Wollocko, J., & Wollocko, H. (2010). Structural and redox behavior of oxyvitaTM, a zero-linked polymeric hemoglobin: Comparison with natural acellular polymeric hemoglobins. Artificial Cells, Blood Substitutes, and Biotechnology, 38(2), 64–68. https://doi.org/10.3109/10731191003634562

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free