In Vitro: Sunscreen Activity of Red Lettuce Extract (Lactuca sativa var. crispa L.)

Abstract

This study aims to determine the activity of sunscreen with the SPF (Sun Protection Factor) value of red lettuce extract (Lactuca sativa var. crispa L.) in various solvents. Red lettuce samples used were obtained from Turirejo village, Lawang district, Malang. Simplicia red lettuce was extracted by maceration method for 24 hours using a various solvents which are methanol, ethyl acetate, ethanol, and n-hexane solvents. Sunscreen activity testing is done in vitro using a UV-Vis spectrophotometer. The results of the sunscreen activity test of red lettuce ethanol extract (Lactuca sativa var. crispa L.) had the highest SPF value with the ultra protection category (29.505 at a concentration of 800 ppm) compared to methanol, ethyl acetate, and n-hexane extracts. With the SPF value of each extract at concentrations of 200, 400, 600, 800, and 1000 ppm respectively 1.364; 1,941; 2,778; 4,089; and 7,675 (for methanol extracts); 1,449; 2,341; 3,868; 6,222; and 10,266 (for ethyl acetate extracts); 2,049; 2,381; 9,455; 29.505; and 35,698 (for ethanol extract); and 1,103; 1,294; 1,322; 1,379; and 1,983 (for n-hexane extract).