Mid-Infrared Spectroscopic Monitoring of Animal Cell Culture Bioreactor Processes

Abstract

Mid-Infrared (MIR) spectroscopy in combination with partial least-squares (PLS) regression analysis was used to monitor the concentrations of glucose, lactate, ammonia, and asparagine in situ during bioreactor cultivation of CHO/SSF3 cells. Simple PLS calibration models were established using referenced in situ collected single-beam spectra of one immobilzed culture. The models were applied to another immobilized culture and one suspension culture. In general, glucose predictions were unbiased for both culture types. Application of the lactate model resulted in a biased prediction during the first part of both cultures. Prediction results of the ammonia and asparagine models had both superior performance for the same type culture used in calibration. A negative bias of approximately 1 mM was observed for all the predictions of ammonia in the suspension culture.