Investigation of the low-dose response in the in vivo induction of micronuclei and adducts by acrylamide

47Citations
Citations of this article
24Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Acrylamide is an industrial chemical used in polymer manufacture. It is also formed in foods processed at high temperatures. It induces chromosome aberrations and micronuclei (MN) in somatic cells of mice, but not rats, and mutations in transgenic mice. This study evaluated the low-dose MN response in mouse bone marrow and the shape of the dose-response curve. Mice were treated orally with acrylamide for 28 days using logarithmically spaced doses from 0.125 to 24 mg/kg/day, and MN were assessed in peripheral blood reticulocytes (RETs) and erythrocytes by flow cytometry. Liver glycidamide DNA adducts and acrylamide and glycidamide N-terminal valine hemoglobin adducts were also determined. Acrylamide produced a weak MN response, with statistical significance at 6.0 mg/kg/day, or greater, in MN-RETs and at 4.0 mg/kg/day or greater in MN normochromatic erythrocytes (NCEs). The MN responses at the lower doses were indistinguishable from the concurrent and historical controls. The adducts increased at a much different rate than the MN. When the MN-NCE values were compared to administered dose, the response was consistent with a linear model. However, when hemoglobin or DNA adducts were used as the dose metric, the response was significantly nonlinear, and models that assumed a threshold dose of 1 or 2 mg/kg/day provided a better fit than a linear model. The MN-RET dose-response had greater variability than the MN-NCE response and was consistent with linearity and with a threshold at 1 or 2 mg/kg/day, regardless of the dose metric. These data suggest a threshold for acrylamide in the MN test. © The Author 2008. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved.

References Powered by Scopus

Chemistry, biochemistry, and safety of acrylamide. A review

1106Citations
N/AReaders
Get full text

Chronic toxicity and oncogenicity study on acrylamide incorporated in the drinking water of Fischer 344 rats

388Citations
N/AReaders
Get full text

A lifetime oncogenicity study in rats with acrylamide

336Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Scientific Opinion on acrylamide in food

0
483Citations
N/AReaders
Get full text

Micronucleus assay: The state of art, and future directions

178Citations
N/AReaders
Get full text

The formation and biological significance of N7-guanine adducts

177Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Zeiger, E., Recio, L., Fennell, T. R., Haseman, J. K., Snyder, R. W., & Friedman, M. (2009). Investigation of the low-dose response in the in vivo induction of micronuclei and adducts by acrylamide. Toxicological Sciences, 107(1), 247–257. https://doi.org/10.1093/toxsci/kfn214

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 13

72%

Researcher 3

17%

Professor / Associate Prof. 2

11%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 9

56%

Arts and Humanities 4

25%

Environmental Science 2

13%

Computer Science 1

6%

Save time finding and organizing research with Mendeley

Sign up for free