PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARg

Abstract

Obesity is a global health threat, and the induction of white adipose tissue (WAT) browning presents a promising therapeutic method for it. Recent publications revealed the essential role of protein argininemethyltransferase 4 (PRMT4) in lipidmetabolismand adipogenesis, but its involvement in WAT browning has not been investigated. Our initial studies found that the expression of PRMT4 in adipocytes was upregulated in cold-induced WAT browning but downregulated in obesity. Besides, PRMT4 overexpression in inguinal adipose tissue accelerated WAT browning and thermogenesis to protect against high-fat diet–induced obesity and metabolic disruptions.Mechanistically, our work demonstrated that PRMT4 methylated peroxisome proliferatoractivated receptor-g (PPARg) on Arg240 to enhance its interaction with the coactivator PR domain-containing protein 16 (PRDM16), leading to the increased expression of thermogenic genes. Taken together, our results uncover the essential role of the PRMT4/PPARg/PRDM16 axis in the pathogenesis ofWAT browning.