Lack of association between I/D ACE and -675 ID 4G / 5G PAI-1 polymorphisms and predicting risk of pregnancy loss (PROPALO) in Bosnian women

Abstract

Successful pregnancies, among others, require stabilization of the placental basal plate, preventing accumulation of fibrin in placental vessels and intervillous spaces. Fibrinolysis is regulated by PAI-1, which synthesis is induced by angiotensin II, which is being generated by ACE I. In recent studies, it was found that -675 ID, 4G/5G PAI-1 gene polymorphism, as referred to “a new thrombophilic factor” may increase risk of pregnancy loss. Data on prevalence of genetic risk factors of thrombophilia in Bosnia and Herzegovina (B&H) are rare. Aim: Therefore, we aimed to determine prevalence of I/D ACE (rs l799752) and -675 ID, 4G/5G PAI-1 (rs 1799889) polymorphisms in Bosnian women with pregnancy loss and evaluate the results of the risk of miscarriages. Material and Methods: We prospectively recruited 308 women in total, particulary 154 women with pregnancy loss (PL), mean age 33.0 (±5.4) years and 154 controls without pregnancy loss, at least one liveborn child, mean age 31.4 (±6.7) years. All women were enrolled from Institution of Health Protection of Women and Motherhood (Sarajevo, B&H). Following DNA isolation from buccal swabs, PCR for ACE and real-time PCR for PAI-1 was performed. Results: In women with PL and controls, the frequencies of D and 4G alleles were: 52.3% and 55.5% and 60.1% and 59.7%, respectively. There was no difference of frequency of mutated alleles in group of PL compared to the controls (p>0.05). Conclusion: Our results, did not confirm association of I/D ACE and -675 ID, 4G/5G PAI-1 polymorphisms with pregnancy loss in examined population.