Identification of an extended half-site motif required for the function of peroxisome proliferator-activated receptor α

48Citations
Citations of this article
9Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Background: Peroxisome proliferator-activated receptors (PPARs) belong to the nuclear hormone receptor superfamily and regulate many genes of the proteins involved in lipid metabolism, including peroxisomal acyl-CoA oxidase (AOX). Through heterodimerization with retinoid X receptors (RXRs), PPAR was believed to recognize the sequence elements consisting of two directly repeating 6-bp half-sites spaced by one nucleotide (DR-1), located in the regulatory regions of these genes. Results: Employing the peroxisome proliferator-responsive enhancer of the rat AOX gene, we analysed the minimal sequence requirements for enhancer activity and PPARα/RXRα binding. We found that the sequence just downstream of the DR-1 motif is indispensable for both functions. By a direct selection procedure of high-affinity binding sites from a random sequence pool, we identified a consensus sequence at the four positions next to DR-1. We also suggest that PPARα binds to the downstream half-site, whereas RXRα binds to the upstream half-site of the AOX DR-1. Conclusions: An extended half-site of 10-bp, but not a simple 6-bp half-site, is required for the PPARα binding, upon heterodimer formation with RXRα. The binding polarity of PPARα/RXRα seems to be opposite to that of other RXR-involving heterodimers. © Blackwell Science Limited.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Osada, S., Tsukamoto, T., Takiguchi, M., Mori, M., & Osumi, T. (1997). Identification of an extended half-site motif required for the function of peroxisome proliferator-activated receptor α. Genes to Cells, 2(5), 315–327. https://doi.org/10.1046/j.1365-2443.1997.1220319.x

Readers over time

‘13‘15‘20‘21‘22‘2400.511.52

Readers' Seniority

Tooltip

Professor / Associate Prof. 2

29%

PhD / Post grad / Masters / Doc 2

29%

Researcher 2

29%

Lecturer / Post doc 1

14%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 5

71%

Biochemistry, Genetics and Molecular Bi... 2

29%

Save time finding and organizing research with Mendeley

Sign up for free
0