Phosphatidylinositol 3-kinase activity negatively regulates stability of cyclooxygenase 2 mRNA

83Citations
Citations of this article
26Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Human alveolar macrophages have both lipopolysaccharide (LPS)-induced and constitutive phosphatidylinositol 3-kinase (PI3K) activity. We observed that blocking PI3K activity increased release of prostaglandin E2 after LPS exposure, and increasing PI3K activity (interleukin-13) decreased release of prostaglandin E2 after LPS exposure. This was not because of an effect of PI3K on phospholipase 2 activity. PI3K inhibition resulted in an increase in cyclooxygenase 2 (COX2) protein, mRNA, and mRNA stability. PI3K negatively regulated activation of the p38 pathway (p38, MKK3/6, and MAPKAP2), and an active p38 was necessary for COX2 production. The data suggest that PI3K inhibition of p38 modulates COX2 expression via destabilization of LPS-induced COX2 mRNA.

References Powered by Scopus

Innate immune recognition

6661Citations
N/AReaders
Get full text

Toll-like receptors and innate immunity

3472Citations
N/AReaders
Get full text

A protein kinase involved in the regulation of inflammatory cytokine biosynthesis

3218Citations
N/AReaders
Get full text

Cited by Powered by Scopus

PI3K and negative regulation of TLR signaling

550Citations
N/AReaders
Get full text

Molecular mechanism of endotoxin tolerance

376Citations
N/AReaders
Get full text

M2-polarized tumor-associated macrophages promoted epithelial-mesenchymal transition in pancreatic cancer cells, partially through TLR4/IL-10 signaling pathway

375Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Monick, M. M., Robeff, P. K., Butler, N. S., Flaherty, D. M., Brent Carter, A., Peterson, M. W., & Hunninghake, G. W. (2002). Phosphatidylinositol 3-kinase activity negatively regulates stability of cyclooxygenase 2 mRNA. Journal of Biological Chemistry, 277(36), 32992–33000. https://doi.org/10.1074/jbc.M203218200

Readers over time

‘11‘12‘13‘14‘15‘16‘17‘18‘19‘20‘21‘22‘2302468

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 13

68%

Professor / Associate Prof. 4

21%

Researcher 2

11%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 9

50%

Biochemistry, Genetics and Molecular Bi... 4

22%

Medicine and Dentistry 3

17%

Immunology and Microbiology 2

11%

Save time finding and organizing research with Mendeley

Sign up for free
0