Simultaneous expression of stilbene synthase genes in Japanese red pine (Pinus densiflora) seedlings

Abstract

We analyzed sequences of the stilbene synthase (STS) cDNA in Pinus densiflora. Three novel STS cDNA clones (pdsts1, pdsts2, pdsts3) that carry full coding sequences were isolated from a cDNA library constructed from the roots. The homologies in their coding regions were about 95%, and they have a conserved STS motif. Their phylogenetic relation was also discussed. The 3′ end of the STS cDNA fragments was amplified by the polymerase chain reaction and subcloned. The 3′ untranslated region (3′UTR) of the fragments highlighted the structure diversity. Twelve STS cDNA clones were categorized into seven distinct subclasses according to 3'UTR sequences. We discussed the stability of the transcripts and their gene organization. The simultaneous expression of the STS members is one of the mechanisms for adaptation that pine trees have developed over time.