Crystallization and preliminary crystallographic studies of the D59A mutant of MicA, a YycF response-regulator homologue from Streptococcus pneumoniae

Abstract

RR02 (MicA) is an essential bacterial protein that belongs to the YycF family of response regulators and consists of two domains: an N-terminal receiver-domain and a C-terminal effector domain. Streptococcus pneumoniae RR02 (MicA; residues 2-234) has been crystallized using the sitting-drop vapour-diffusion technique. The crystals belong to space group P21, with unit-cell parameters a = 46.46, b = 32.61, c = 63.35 Å, β = 90.01°. X-ray diffraction data have been collected to 1.93 Å resolution. © 2004 International Union of Crystallography. Printed in Denmark - all rights reserved.