Crystallization and Preliminary X-ray Analysis of GAP 31. A protein Which Inhibits the Life Cycle of HIV-1

Abstract

GAP 31 is an anti-HIV plant protein that we have identified and purified to homogeneity from Gelonium multiflorum. It is the first reported example of an anti-HIV agent capable of acting against multiple stages of the viral life cycle, on viral infection and viral replication. GAP 31 is a unique paragon of multi-functional protein. In addition to anti-HIV activity, it also exhibits anti-tumor action, DNA binding, RNA binding and ribosome inactivation. The present crystals diffract up to 2·0 Å resolution and belong to monoclinic space group P21. The cell dimensions are a = 49·30(2) Å, b = 44·57 Å, c = 137·78(7) Å and β = 98·32(3)°. There are two molecules of molecular weight 31 kDa in an asymmetric unit with a solvent content of 49%. © 1994 Academic Press Limited.