Effect of oral administration of omeprazole on the microbiota of the gastric glandular mucosa and feces of healthy horses

Abstract

Background: Omeprazole administration is associated with changes in gastric and fecal microbiota and increased incidence of Clostridioides difficile enterocolitis in humans and dogs. Hypothesis/Objectives: Study purpose was to assess the effect of omeprazole on gastric glandular and fecal microbiota in healthy adult horses. Animals: Eight healthy horses stabled on straw and fed 100% haylage. Methods: Prospective controlled study. Transendoscopic gastric glandular biopsies, gastric fluid, and fecal samples were obtained from each horse twice at a 7-day interval before the administration of omeprazole. Samples were taken on the same horses before and after a 7-day administration of omeprazole (4 mg/kg PO q24h). pH was assessed on fresh gastric fluid and other samples were kept at −20°C until analysis. Bacterial taxonomy profiling was obtained by V1V3 16S amplicon sequencing from feces and gastric glandular biopsies. Analysis of alpha, beta diversity, and comparison between time points were performed with MOTHUR and results were considered significant when P