Antioxidant activity of Diadema setosum gonads from Kelapa island, district of Seribu island, Indonesia

Abstract

Oxidative damage caused by ROS (Reactive Oxygen Species) can trigger various chronic diseases. Marine antioxidant research has focused mainly on the antioxidant effects of crude extracts. This study aims to examine the antioxidant effect of polar and non-polar fractions of Diadema setosum gonads fraction. Sea urchin samples were obtained from Kelapa Island, Indonesia. The gonads of D. setosum were harvested, cut into small pieces, ground and macerated with methanol in a ratio of 1: 3 w/v to the mass of the sample. The methanol filtrate was concentrated with a rotary evaporator at 40oC until a dry mass was obtained. The crude extract was fractionated in n-hexane, ethyl acetate and water, respectively. The crude methanol extract and fractions were tested for antioxidant activity using the DPPH free radical scavenging assay. The results showed that the n-hexane fraction had the highest antioxidant activity compared to the ethyl acetate and water fractions with IC50 value of 77.10 ppm.