The liver performs a number of physiologically important functions. Hepatocytes are the liver parenchymal cells performing most of those functions. Therefore, it is important to recover functional hepatocytes after hepatic injury and prepare a mass of hepatocytes for regenerative medicine. We have found that mature hepatocytes dedifferentiate to hepatocyte progenitors in chronically injured mouse liver. Those hepatocyte progenitors can be isolated as CD24+EpCAM− cells from the CD31−CD45− fraction, which clonally proliferate and efficiently re-differentiate to functional hepatocytes both in vitro and in vivo. Here, I describe the methods to isolate hepatocyte progenitors from chronically injured liver, to expand them in vitro, and to induce differentiation into functional hepatocytes.
CITATION STYLE
Tanimizu, N. (2019). Identification and in vitro expansion of adult hepatocyte progenitors from chronically injured livers. In Methods in Molecular Biology (Vol. 1940, pp. 267–273). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9086-3_19
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