ROLE OF DIFFERENT CARBON SOURCES ON IN VITRO MICROPROPAGATION OF OXALIS CORNICULATA (L.)

Abstract

The response towards nodal explants of O.Corniculata with regard to multiple shoot regeneration was experienced on media containing different carbohydrates Sucrose, Fructose, Maltose and Glucose. The substitution of these sugars shown different responses in different concentrations. Data was collected in terms of regeneration frequency and growth rate. The plant growth regulators BAP and NAA are used on MS Media containing various carbon sources. Among all the concentrations of carbohydrates (1-6%) studied, fructose (2%) showed best performance with maximum shoots (24.4±0.2) and shoot length (13.6±0.1). And also shoot regeneration and shoot multiplication appreciably increased in fructose. This was in line with sucrose, maltose and glucose. The least number of shoots was observed in glucose (10.5±0.4) and the least shoot length (4.4±0.2) with very stunted growth was obtained in maltose. This results noticeably indicates that fructose (2%) is proved to be best for multiple shoot regeneration from nodal explants of O.corniculata. The best response for rooting was obtained in MS Medium supplemented with IBA at 2.0 mg/l.