Analysis of the C. Elegans nucleolus by immuno-DNA fish

Abstract

Caenorhabditis elegans is a well-established model organism which allows, among others, to investigate the link between nucleolar structure/function on the one hand and cell fate choices and cellular differentiation on the other. In addition, C. elegans can be used to study the role of the nucleolus in processes that can be diffi cult to faithfully reproduce in vitro, such as gametogenesis, disease development, and aging. Here I present two complementary techniques, immunofl uorescent staining and DNA fl uorescence in situ hybridization, that have been adapted to label nucleolar components at various stages of the life cycle of the worm.