A commercialized photoautotrophic micropropagation system using large vessels with forced ventilation

Abstract

A photoautotrophic micropropagation system (called a PAM hereafter) that uses a sugar-free culture medium has many advantages over the conventional photomixotrophic micropropagation system (called a PMM hereafter) that utilizes a sugar-containing culture medium (Kozai, 1991). The advantages include the use of large culture vessels with minimum risk of microbial contamination and the enhancement of plantlet growth at a high photosynthetic photon flux (PPF) and a high CO2 concentration inside the vessel (Fujiwara et al., 1988; Kozai and Iwanami, 1988). © 2005 Springer. All Rights Reserved.