Antibiotic Resistant Pattern of K. pneumoniae and their Biofilm Development on Diverse Surfaces

Abstract

The objective of the study is to determine the antibiotic resistance and biofilm formation of Klebsiella pneumoniae in different surfaces. In this study, 47 K. pneumoniae strains were procured from K.A.P. Viswanatham Government Medical College, Trichy, Tamil Nadu. All the isolates were reconfirmed through biochemical reactions. Maximum resistance was observed against Ampicillin, Cefepime, Cefotaxime, Co-trimoxazole, Aztreonam, and Imipenem. The lowest resistance was noticed against Cefuroxime. Among these 85% strains exhibited multidrug resistance with 13% was carbapenem and 98% eSBL resistant strains. The biofilm formation of all the strains in different surfaces revealed that stainless steel surface found to be adhered high number of cells than in other surfaces. Similarly, the biofilm formation of strains grown with glucose in tryptic soya broth (TSB) enhanced adherence ability. It is concluded that presence of glucose or any sugar substrates enhances the biofilm formation thereby developing high resistance against different antibiotics. This condition is detrimental to human health and causes considerable concern.