HSV-1 amplicon vectors have been used as platforms for the generation of genetic vaccines against both DNA and RNA viruses. Mice vaccinated with such vectors encoding structural proteins from both foot-and-mouth disease virus and rotavirus were partially protected from challenge with wild-type virus (D’Antuono et al., Vaccine 28:7363–7372, 2010; Laimbacher et al., Mol Ther 20:1810–1820, 2012; Meier et al., Int J Mol Sci 18:431, 2017), indicating that HSV-1 amplicon vectors are attractive tools for the development of complex and safe genetic vaccines. This chapter describes the preparation and testing of HSV-1 amplicon vectors that encode individual or multiple viral structural proteins from a polycistronic transgene cassette. We further put particular emphasis on generating virus-like particles (VLPs) in vector-infected cells. Expression of viral genes is confirmed by Western blot and immune fluorescence analysis and generation of VLPs in vector-infected cells is demonstrated by electron microscopy. Furthermore, examples on how to analyze the immune response in a mouse model and possible challenge experiments are described.
CITATION STYLE
Meier, A. F., & Laimbacher, A. S. (2020). HSV-1 Amplicon Vectors as Genetic Vaccines. In Methods in Molecular Biology (Vol. 2060, pp. 111–130). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9814-2_6
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