Monitoring of Extracellular TCA Cycle Intermediates in Mammalian Cell Culture

Abstract

Some intracellular intermediate metabolites can also be found in the medium supernatant at micromolar concentrations. In this work, we are investigating extracellular concentrations of five organic acids (succinic, malic, fumaric, citric and isocitric acid) during cell growth and after viral infection of MDCK cells.