Preliminary study on salt resistance seedling trait in maize by SRAP molecular markers

Abstract

In this study, different genotypes of maize salt tolerance inbred line and salt sensitive inbred line were used as the parent hybrid combinations to obtain F2 populations. Two salt tolerance extreme types of DNA pools were established, where BSA method was used to select polymorphic SRAP markers. The result showed that 48 pair primers can be amplified and clear and stable bands can be obtained by parental, tolerant, and sensitive gene pools. Six pair primers of M2E1, M2E7, M6E15, M7E7, M11E4, and M14E6 showed polymorphism between two parents and between tolerant and sensitive bulks. The six SRAP molecular markers closely linked to salt tolerance were determined. The best maize SRAP-PCR reaction system was established. This research will accelerate maize markerassisted selection breeding and lay the foundation for salt-tolerant gene cloning.