Primer screening and genetic diversity analysis of jabon putih (Anthocephalus cadamba (roxb) miq.) based on random amplified polymorphic dna (rapd) markers

Abstract

Introduction: Jabon putih population has currently decreased because of intensive exploitation and land use conversion. Genetic diversity is an essential factor in maintaining the existence of a species. Objective: The objective of this study was to determine the genetic relationship in Jabon putih from the genetic resource area of the second region of Seed/Seedling Forest Office in Bellabori, Parangloe, Gowa, based on RAPD markers. Methods: The analysis was carried out using 145 DNA samples from seven populations. Primer screening using randomly selected twelve DNA observed three polymorphic primers out of 21 screened RAPD primers. Result: The polymorphic primers were OPP-08, OPY-09, and OPD-20. The mean of heterozygosity was 0.46, and that of the highest was detected in Luwu Population and Wajo Population (0.48, respectively). Conclusion: The individuals tended to randomly group (did not group according to their provenances), and consequently, the genetic diversity among populations is high.