The enzyme systems in a strain of candida wickerhamii meyer and yarrow participating in the hydrolysis of cellodextrins

Abstract

Two β-glucosidases from Candida wickerhamii were studied. One is parietal and exocellular, the other is endocellular. Both enzymes were purified by ion-exchange chromatography and gel filtration. They could hydrolyze glucosides with β (l-3), β (1–4) and β (l-6) configurations. The exocellular enzyme is active against soluble cellodextrins (D.P 3 to 6). This is responsible for the ability of the Candida wickerhamii strain to ferment soluble cellodextrins. Both enzymes were constitutive, but their biosynthesis was repressed by glucose. © 1984, Applied Microbiology, Molecular and Cellular Biosciences Research Foundation. All rights reserved.