Genetic Diversity Based on RAPD Marker of Ramie Plants (Boehmeria nivea [L.] Gaud) in West Sumatera

Abstract

This research has been conducted at the Experimental Farm of Agricultural Faculty of Andalas University, two districts in West Sumatera (Lima Puluh Kota and Agam) and the Laboratory of the Department of Agronomy and Horticulture Agricultural Faculty, Bogor. The purpose of this study was to determine the genetic diversity of the ramie plants collection Experimental Farm, Agricultural Faculty of Andalas University and exploration at two districts in West Sumatera. This research was conducted by molecular characterization, using RAPD markers (Random Amplified Polymorphic DNA) with 5 primers (OPF 4, OPF5, OPH7, OPX2, and OPX 17). The samples used were 4 clones of ramie plants (Padang 3, Ramindo, Matur and Situjuah) with 3 replications. Fragments were sized with reference to the size markers and scored (present/absent). The similarity coefficients calculated and dendograms produced using NTSYS software (version 2.02). From the results of the study the results of the similarity level coefficient were 0.56-0.78, and formed 2 large groups (I, II). M1 and M2 clones are in group I, while in groups II (IIa and IIb). Group IIa (R1, R2, R3, P3, M1) and Group IIb (S1, S2, S3, P1).