Multiplex Immunoassay: A Planar Array on a Chip using the Magarray TM Technology

Abstract

Multiplexing is an important tool in assay development as it allows simultaneous detection of numerous analytes. Current platforms with the capability to multiplex are often complex and expensive. Here, we describe a low-cost planar array on a chip capable of simultaneously detecting up to 80 different analytes using the MagArray technology in as little as 10 min. This technology is easy to operate, has a small footprint, and is highly portable as it does not require any moving parts and/or microfl uidics. This technology also allows the user to obtain a real-time read-out, which is very useful for analyzing complex sample matrices and for assessing cross-reactivity easily, or to monitor the dissociation of low affi nity proteins during washes. The recommended sample volume for analysis is 100 μL after dilution, but as little as 20 μL can be measured if needed.