In Escherichia coli, conventional amber and ochre stop codons can be separately targeted by engineered amber-suppressing Methanocaldococcus jannaschii tyrosyl-tRNA synthetase-tRNAPyl and ochre-suppressing Methanosarcina maezi pyrrolysyl-tRNA synthetase-tRNAPyl pairs for coding two different noncanonical amino acids in one protein gene. Here, we describe the application of this approach to produce a protein with two distinct chemical functionalites which can be selectively labeled with two fluorescent dyes.
CITATION STYLE
Tharp, J. M., & Liu, W. R. (2018). Using amber and ochre nonsense codons to code two different noncanonical amino acids in one protein gene. In Methods in Molecular Biology (Vol. 1728, pp. 147–154). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7574-7_9
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