Molecular identification of inulinolytic yeast isolated from cherry fruit (Muntingia calabura L.) based on internal transcribed spacer sequence

Abstract

Inulinolytic yeast is a yeast that produces inulinase enzyme (E.C.3.2.1.7). Inulinase has an important role to hydrolyze inulin into simpler molecules that are widely used in industry. Previous research has obtained K4 inulinolytic yeast from cherry fruit (Muntingia calabura L.) which has not been identified yet. This study aims to molecularly identify and phylogenetic analysis of K4 inulinolytic yeast isolated from cherry fruit (M. calabura L.) based on the Internal Transcribed Spacer (ITS) sequence. Research stages consist of DNA isolation, amplification of the ITS rDNA sequence, electrophoresis of PCR product, analysis of sequence data, and phylogenetic analysis using MEGA X. The results showed that yeast DNA was successfully obtained with a concentration of 807.1 ng/µL and had a purity value of 2.12. Amplification of the ITS rDNA sequence produced amplicon with length 250 bp. Molecular identification based on homology analysis with database of The National Center for Biotechnology Information (NCBI) and phylogenetic analysis indicated that K4 isolate was the most closely related to Diutina rugosa isolate S217 (MF797783).