Microbroth dilution susceptibility testing of Candida species

Abstract

Antifungal susceptibility testing for Candida species is now widely accepted as a methodology to predict the success or failure of antifungal therapy for some antifungal/ Candida species combinations. There are many different ways to perform susceptibility testing of antifungal agents, but broth microdilution has become the most popular over the last 10 years. This chapter describes in detail two methods for antifungal susceptibility testing of Candida species using the commercially available microbroth dilution tray (YeastOne®) and a commercially available gradient agar diffusion technique (Etest®) for isolates that appear resistant.