Abstract
In mammals, the introduction of the OSKM (Oct4, Sox2, Klf4, and c-Myc) genes into somatic cells has allowed generating induced pluripotent stem (iPS) cells. So far, this process has been only clearly demonstrated in mammals. Here, using chicken as an avian model, we describe a set of protocols allowing the establishment, characterization, maintenance, differentiation, and injection of putative reprogrammed chicken Induced Pluripotent Stem (iPS) cells.
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Fuet, A., & Pain, B. (2017). Chicken induced pluripotent stem cells: Establishment and characterization. In Methods in Molecular Biology (Vol. 1650, pp. 211–228). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7216-6_14
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