Molecular characterization of the resistance of Mycobacterium tuberculosis to second line drugs in Côte d'ivoire

Abstract

Purpose: To characterize the resistance of Mycobacterium tuberculosis to second line drugs using a line probe assay. Methods: Multi-drug resistant strains of Mycobacterium tuberculosis isolated between December 2008 and December 2009 were tested for resistance to fluoroquinolones and second-line injectable drugs using GenoType® MTBDRsl. Results: Thirty eight strains gave interpretable results. None of them had a mutation in the gyrA gene. Regarding second-line injectable drugs, 4 strains (11%) were resistant to aminoglycosides/ capreomycin and all of them harbored A1401G mutation. Conclusion: No extensive drug resistant strains were observed. A relatively high proportion of strains were resistant to at least one second-line injectable drug. Resistance mechanism seemed similar for all of them. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.