One-and-a-half wavelength approach

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Abstract

In many cases single-wavelength anomalous diffraction (SAD) phasing leads to a successful structure solution, but it is impossible to predict beforehand if single-wavelength data with a certain amount of anomalous signal resulting will be sufficient. It is therefore safer to continue collecting data at different wavelengths according to a MAD protocol, but to simultaneously attempt to phase the first data set by the SAD method. If this is successful, then further data collection can be abandoned. This '1.5-wavelength' approach may save a substantial amount of time and effort and diminishes the effects of crystal radiation damage. The principles of SAD phasing are illustrated using vector diagrams in the Argand plane.

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APA

Dauter, Z. (2002). One-and-a-half wavelength approach. In Acta Crystallographica Section D: Biological Crystallography (Vol. 58, pp. 1958–1967). https://doi.org/10.1107/S0907444902016645

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