Posttranscriptional regulation of PTEN by competing endogenous RNAs

Abstract

PTEN expression can be dysregulated in cancers via multiple mechanisms including genomic loss, epigenetic silencing, transcriptional repression, and posttranscriptional regulation by microRNAs. MicroRNAs are short, noncoding RNAs that regulate gene expression by binding to recognition sites on target transcripts. Recent studies have demonstrated that the competition for shared microRNAs between both protein-coding and noncoding transcripts represents an additional facet of gene regulation. Here, we describe in detail an integrated computational and experimental approach to identify and validate these competing endogenous RNA (ceRNA) interactions.